Composite

Part:BBa_K5117039

Designed by: Jenny Sauermann, Lilli Kratzer, Katrin Lehmann   Group: iGEM24_TU-Dresden   (2024-09-28)
Revision as of 12:08, 1 October 2024 by Lilli-kratzer (Talk | contribs)


PcotYZ-BsRBS-BhBglA-L2-CotY-B0014

This part serves as transcriptional unit composed of:

  • promoter PcotYZ of Bacillus subtilis (BBa_K5117021),
  • ribosome binding site of Bacillus subtilis (BBa_K5117000)
  • bglA gene of Bacillus halodurans encoding a beta-glucosidase (EC 3.2.1.21),
    • addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 (BBa_K5117027),
  • cotY gene of Bacillus subtilis (BBa_K5117022),
  • bidirectional terminator B0014 (BBa_B0014).


    • Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      INCOMPATIBLE WITH RFC[12]
      Illegal NheI site found at 764
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      INCOMPATIBLE WITH RFC[25]
      Illegal NgoMIV site found at 1624
    • 1000
      COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None