Composite

Part:BBa_K200018:Experience

Designed by: Royah Vaezi   Group: iGEM09_Imperial College London   (2009-10-15)
Revision as of 00:27, 22 October 2009 by Kun (Talk | contribs) (→‎User Reviews)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K200018

User Reviews

UNIQ4f43c7a94ae29b9e-partinfo-00000000-QINU UNIQ4f43c7a94ae29b9e-partinfo-00000001-QINU

UNIQ4f43c7a94ae29b9e-partinfo-00000002-QINU

BBa_K200018 1 Not understood Kun Xue

II09 CRP-GFP fluor different media.jpg

Cells with BBa_K200018 were grown overnight in various different media, and the GFP fluorescence was measured.

After overnight culture, the corrected fluorescence of glucose is almost negligible, showing that glucose represses the PcstA promoter strongly.

For all the other secondary carbon sources, 10% Casamino Acids in M9 shows the highest corrected fluorescence at 22000.

II09 CRP-GFP abs different media.jpg

For effective gene expression, not only must the promoter activity be strong, but there must also be enough cells expressing the genes. Therefore, OD600 is also a critical factor.

Due to the exceedingly low OD600 in 0.5% Lactose in M9, the medium cannot be used even though it induces relatively high fluorescence.

For more information, go to the Imperial iGEM 2009 E.ncapsulator project page on [http://2009.igem.org/Team:Imperial_College_London/Wetlab/Results/CRP_and_Media BBa_K200018 testing results]


;

UNIQ4f43c7a94ae29b9e-partinfo-00000004-QINU