Part:BBa_K200018:Experience
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BBa_K200018 1 Not understood Kun |
Cells with BBa_K200018 were grown overnight in various different media, and the GFP fluorescence was measured. For effective gene expression, not only must the promoter activity be strong, but there must also be enough cells expressing the genes. Therefore, OD600 is also a critical factor.
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[http://2012.igem.org/Team:University_College_London The 2012 iGem UCL team] further characterized the cstA promoter (Part:BBa_K118011) using GFP as a reporter gene (Part:BBa_E0040). E. coli transformed with cstA promoter fused with GFP was grown overnight in LB media, in order to induce the starvation response cells were centrifuged and resuspended in minimal M9 media supplemented with different glucose concentrations (0%, 0.2%, 0.5%, 1.0%, 2.0%, 5%) and chloramphenicol (15 μg/mL). The tested cultures incubated at different concentrations of glucose shown a trend of decreasing GFP expression with raising glucose concentration. Fluorescence was highest at 0% added glucose and lowest at 5%, which implies that the starvation response increases the cstA promoter activity. UNIQa9dbaf7b09c3bc3b-partinfo-00000004-QINU |