Part:BBa_K5115085

F1v (FKBP with F36V mutation) without stop codon

Introduction

This part is FKBP with F36V mutation, which is a signal transducer. Binding with F1v, the target protein can get dimerized with B/B Homodimerizer in the culture solution.

Usage and Biology

In our experiment, we adopted the iDimerize kit, to learn fully about this kit, please check iDimerize™ Inducible Homodimer System User Manual

In BBa_K5115086(NixA-F1v) and BBa_K5115087(F1v-NixA), we link this part with BBa_K5115071(NixA) in two different ways to find the more efficient way of dimerizing the NixA protein.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]