Coding

Part:BBa_K5115071

Designed by: Yi Shi   Group: iGEM24_Fudan   (2024-09-19)
Revision as of 03:17, 28 September 2024 by Chenliyue (Talk | contribs)


NixA, without stop codon

contributed by Fudan iGEM 2023

Introduction

NixA is a high-affinity nickel transporter in helicobacter pylori. It belongs to the NiCoT family of transporters and facilitates the import of Ni²⁺ ions across the bacterial cytoplasmic membrane. These nickel ions make up the active sites of urease and hydrogenase, enzymes crucial for H. pylori's ability to survive in the highly acidic environment of the stomach.[1]

Usage and Biology

We delete the stop codon of this part to link it with BBa_K5115085(F1v).For more information about their experiment, please check BBa_K5115086(NixA-F1v)and BBa_K5115087(F1v-NixA).

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. ↑ Fischer, F., Robbe-Saule, M., Turlin, E., Mancuso, F., Michel, V., Richaud, P., Veyrier, F. J., Reuse, H. D., & Vinella, D. (2016). Characterization in Helicobacter pylori of a Nickel Transporter Essential for Colonization That Was Acquired during Evolution by Gastric Helicobacter Species. PLOS Pathogens, 12(12), e1006018.
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