Part:BBa_K5477022
POR - Cytochrome P450 oxidoreductase codon optimized for Saccharomyces cerevisiae
Cytochrome P450 oxidoreductase (POR) is an essential enzyme that provides the necessary electrons to support the function of cytochrome P450 enzymes in their metabolic activities. POR is a flavoprotein, containing both FMN (flavin mononucleotide) and FAD (flavin adenine dinucleotide) cofactors, which are critical for transferring electrons from NADPH to cytochrome P450 enzymes. This electron transfer is necessary for the P450 enzymes to carry out their role in phase I metabolism, specifically the oxidation of a wide variety of substrates, including drugs, toxins, and endogenous compounds. POR is localized in the endoplasmic reticulum of cells, where it interacts with multiple cytochrome P450 enzymes, including CYP3A4, CYP1A1, and others. By delivering the electrons required for these enzymes to function, POR facilitates the catalytic cycle of cytochrome P450s, which involves the incorporation of molecular oxygen into substrates, often resulting in hydroxylated metabolites. These metabolites are usually more hydrophilic and can be further processed by phase II enzymes, such as UGTs (UDP-glucuronosyltransferases), making them easier to excrete from the body.
In our system, POR plays a critical role in enabling the CYP3A4 and other cytochrome P450 enzymes to function effectively. This part was combined with CYP3A4 or CYP1A1. The following composites and devices, where this part was used, are listed here: | BBa_K5477037, | BBa_K5477038, | BBa_K5477039 and | BBa_K5477047
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 296
Illegal PstI site found at 493
Illegal PstI site found at 877
Illegal PstI site found at 1317
Illegal PstI site found at 1377 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 296
Illegal PstI site found at 493
Illegal PstI site found at 877
Illegal PstI site found at 1317
Illegal PstI site found at 1377 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 296
Illegal PstI site found at 493
Illegal PstI site found at 877
Illegal PstI site found at 1317
Illegal PstI site found at 1377 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 296
Illegal PstI site found at 493
Illegal PstI site found at 877
Illegal PstI site found at 1317
Illegal PstI site found at 1377
Illegal NgoMIV site found at 699
Illegal NgoMIV site found at 818 - 1000COMPATIBLE WITH RFC[1000]
References
1. Pandey AV, Flück CE. NADPH P450 oxidoreductase: structure, function, and pathology of diseases. Pharmacol Ther. 2013;138(2):229-254. doi:10.1016/j.pharmthera.2013.01.010
2. Pandey AV, Sproll P. Pharmacogenomics of human P450 oxidoreductase. Front Pharmacol. 2014 May 9;5:103. doi: 10.3389/fphar.2014.00103. PMID: 24847272; PMCID: PMC4023047.
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