Part:BBa_K5115026

csoS2

Introduction

The csoS2 is a subunit of the csoS operon BBa_K5115034.

Usage and Biology

CsoS2 is a critical protein involved in the assembly of the α-carboxysome, specifically in organizing the shell and encapsulating cargo enzymes. In our system, when combined with these other components, CsoS2 ensures the correct formation of the empty carboxysome structure and plays a crucial role in incorporating target enzymes inside the shell. Together with the other csoS subunits, CsoS2 helps create a functional, enclosed environment for biotechnological applications, such as enzyme encapsulation for improved catalytic efficiency.

Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 133
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 291
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 799
  Illegal AgeI site found at 1750
  Illegal AgeI site found at 2431
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 191