Part:BBa_K5317018
CMV-EGFP-PknB
Usage and Biology
PknB is a eukaryote-like serine/threonine kinase in Staphylococcus aureus that plays an important role in the bacterial response to antibiotics, particularly beta-lactams, via its PASTA domain (Stehle et al.2012). PknB is a membrane-localized protein consisting of an N-terminal cytosolic kinase domain, a central transmembrane segment and three C-terminal extracellular PASTA domains. The PASTA (penicillin-binding protein and serine/threonine kinase-associated) domain plays a critical role in the recognition and binding of beta-lactam antibiotics (Stehle et al.2012). Upon binding these compounds, the PASTA domain initiates a signaling cascade by inducing autophosphorylation of the N-terminal kinase domain. This activation leads to the initiation of downstream signaling pathways (Cheung et al.2010). In S. aureus, this mechanism is critical for early detection of antibiotics and helps the bacteria adapt to antibiotic stress (Sauer et al. 2018). The composite part includes the upstream positioned reporter gene EGFP (K3338006) to charactarize the PknB regarding it's cellular localization pre and post antibiotics stimulation.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 2596
Illegal SpeI site found at 3259
Illegal PstI site found at 2021 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 3259
Illegal PstI site found at 2021 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2271
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 2596
Illegal SpeI site found at 3259
Illegal PstI site found at 2021 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 2596
Illegal SpeI site found at 3259
Illegal PstI site found at 2021 - 1000COMPATIBLE WITH RFC[1000]
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