Reporter

Part:BBa_K3338006

Designed by: Jonas Scholz   Group: iGEM20_Hannover   (2020-10-23)


EGFP

EGFP is a constitutively fluorescent (enhanced) green fluorescent protein derived from Aequorea Victoria first published by Cormack et al. in 1996 with an excitation wavelength of 488 nm and an emission peak at 507 nm. Due to the introduction of a set of point mutations including a mutation effecting folding efficiency under cell culture conditions, it shows a much higher extinction coefficient and brightness than the natural variant of the protein (Cormack et al. 1996, McRae et al. 2005).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Cormack, B. P., Valdivia, R. H., & Falkow, S. (1996). FACS-optimized mutants of the green fluorescent protein (GFP). Gene, 173(1 Spec No), 33–38.

McRae, S. R., Brown, C. L., & Bushell, G. R. (2005). Rapid purification of EGFP, EYFP, and ECFP with high yield and purity. Protein expression and purification, 41(1), 121–127.

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