Coding

Part:BBa_K5049002

Designed by: Wei Lai   Group: iGEM24_Mingdao   (2024-08-29)
Revision as of 03:41, 10 September 2024 by Yorushika (Talk | contribs)


GS-Pir1 (Pir1 anchor protein with a GS linker)

The sequence of DNA elements for Pir1 anchor protein were from Saccharomyces cerevisiae. To ensure flexibility in connecting to target fusion proteins, a GS linker ((GGSG)3) was added at the N-terminus of Pir1.The GS-Pir1 DNA fragment was synthesized by IDT and cloned into pSB1C3 as a basic part.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 517
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None