Part:BBa_K5049002

GS-Pir1 (Pir1 anchor protein with a GS linker)

Pir1, one of the Proteins with internal repeats (Pir) in Saccharomyces cerevisiae, another key anchor protein in addition to GPI, increases the choices for target proteins to be displayed. It is renowned for forming strong covalent bonds with cell wall components, enhancing enzyme durability under various conditions. This robust attachment keeps enzymes active and intact, supporting sustained industrial processes.

The sequence of DNA elements for Pir1 anchor protein were from Saccharomyces cerevisiae. To ensure flexibility in connecting to target fusion proteins, a GS linker ((GGSG)3) was added at the N-terminus of Pir1.The GS-Pir1 DNA fragment was synthesized by IDT and cloned into pSB1C3 as a basic part.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 4
  Illegal AgeI site found at 517
  
• 1000
  COMPATIBLE WITH RFC[1000]