Composite
His-FluA-S

Part:BBa_K243010

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-14)
Revision as of 19:24, 20 October 2009 by Timo (Talk | contribs)

His-FluA-Split Linker-Fok_i

These part units some of our parts. The combination of a Histidin-Tag, a Anticalin-Tag and the linked protein domain Fok_a, makes it possible to purifier and detect the function of the protein. The fluoresceinA tag allows the measurement by [quenching] and the coupling to an flurescein linked oligo.

Purification

We purified this part of our universal endonuclease by a Histidin column purification and proved it with a following Western Blot.

WB09.10.02jpg.jpg

Western Blot: His-FluA-SplitLi-Fok_i in pEx;
lanes: NEB prestained marker broad range,
pool of elution fractions 2-5, empty lane, 3 positive controls


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 272
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None