![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4825015
Designed by: Ye Siyi Group: iGEM23_GreatBay-SCIE (2023-10-11)
ARO10
ARO10 is derived from S.cerevisiae genome. It encodes for a 2-ketoacid decarboxylase which catalyses the reaction from 2-ketoacid to an aldehyde. It is included in our composite part with ADH7 (alcohol dehydrogenase) and ATF1 (alcohol acetyltransferase), which are transformed into the genome of S.cerevisiae simultaneously for overexpression to produce isoamyl acetate.
Usage and Biology
ARO10 is derived from the genome of S.cerevisiae, encoding for a 2-ketoacid decarboxylase. During the fermentation of yeast, it terminates the recursive pathway, which catalyzes the elongation of a 2-ketoacid by one carbon atom, by converting the 2-ketoacid to an aldehyde. Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1791
Illegal SpeI site found at 742
Illegal PstI site found at 620 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1791
Illegal SpeI site found at 742
Illegal PstI site found at 620 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1791
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1791
Illegal SpeI site found at 742
Illegal PstI site found at 620 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1791
Illegal SpeI site found at 742
Illegal PstI site found at 620 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |