Intermediate
Part:BBa_K218004:Design
Designed by: Jeremy Kubik Group: iGEM09_Calgary (2009-10-19)
Terminator followed by constitutive TetR promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The strong terminator is followed by a constitutive promoter so that the transcription of the gene upstream of the terminator is adequately insulated to not influence transcription of the gene downstream of the TetR promoter.
Source
source