Coding
Part:BBa_K4759021:Design
Designed by: Mengrui Tao Group: iGEM23_Jiangnan-China (2023-10-02)
hemB
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 467
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 467
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 467
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 467
- 1000COMPATIBLE WITH RFC[1000]
Design
Recombinant plasmid pETDuet-hemB-hemC-hemD-hemH is expressed in engineered bacteria
Source
A gene coding for porphobilinogen synthase (PBGS),The enzyme catalyzes the asymmetric condensation and cyclization of two 5-aminolevulinate molecules