Coding

Part:BBa_K4759021:Design

Designed by: Mengrui Tao   Group: iGEM23_Jiangnan-China   (2023-10-02)


hemB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 467
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 467
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 467
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 467
  • 1000
    COMPATIBLE WITH RFC[1000]


Design

Recombinant plasmid pETDuet-hemB-hemC-hemD-hemH is expressed in engineered bacteria


Source

A gene coding for porphobilinogen synthase (PBGS),The enzyme catalyzes the asymmetric condensation and cyclization of two 5-aminolevulinate molecules

References

[1] Sultana, N. Microbial biotransformation of bioactive and clinically useful steroids and some salient features of steroids and biotransformation. Steroids 2018, 136. DOI: 10.1016/j.steroids.2018.01.007. [2] Watchmaker, J.; Callaghan, D. J., III; Dover, J. S. Deoxycholic Acid in Aesthetic Medicine. Advances in Cosmetic Surgery 2020, 3 (1), 77-87. DOI: 10.1016/j.yacs.2020.01.009