Part:BBa_K4765008
INPNC
Introduction
INPNC is a kind of modified INP with truncation of the entire internal repeating domain. INPNC does not appear to be hampered by the size of the passenger while also exhibiting compatibility with the translocation and surface display of proteins containing multiple cofactors and disulfide bond-containing passengers [1].
Usage and Biology
At the C-terminus of INPNC+linker, specific functional proteins can be attached to enable the surface display of it. In our project, we constructed BBa_K4765107(ribozyme+strong RBS+INPNC-Ag3 fusion+stem-loop) and https://parts.igem.org/Part:BBa_K4765108 BBa_ K4765108(ribozyme+strong RBS+INPNC-Nb3 fusion+stem-loop)] for the surface display of Ag/Nb3 and compared it with K4765105 BBa_ K4765105(ribozyme+strong RBS+intimin-Ag3 fusion+stem-loop), K4765106 BBa_ K4765106(ribozyme+strong RBS+intimin-Nb3 fusion+stem-loop), and selected intimin as our surface display system.
Characterization
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 481
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 330
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 72
Illegal NgoMIV site found at 405
Illegal AgeI site found at 429 - 1000COMPATIBLE WITH RFC[1000]
Reference
- ↑ van Bloois, E., Winter, R. T., Kolmar, H., & Fraaije, M. W. (2011). Decorating microbes: Surface display of proteins on Escherichia coli. Trends in Biotechnology, 29(2), 79–86. https://doi.org/10.1016/j.tibtech.2010.11.003
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