Part:BBa_K203111

Constitutive promoter; 2 REU

A constitutive promoter from the Heidelberg 2009 library of promoters for use in mammalian cells.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Functional Parameters

We generated a library of constitutive promoters by [http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters RA-PCR]. We characterized those promoters by Flow cytometry.

This promoter corresponds to clone S10. We confirmed the initial measurement by an independet technique (image analysis). Its expression strength is approx. 2 [http://2009.igem.org/Team:Heidelberg/Project_Measurement REU].

MIT MAHE 2020

Summary

NF-κB has been considered a prototypical proinflammatory signaling pathway. This is based on the activation of NF-κB by proinflammatory cytokines such as interleukin 1 (IL-1) and tumor necrosis factor α (TNFα). It also has a role in the expression of other proinflammatory genes including cytokines, chemokines, and adhesion molecules. This part contains a synthetic promoter upregulated by NF-κB activation. Manufactured by RA-PCR.

References

1. Lawrence T. (2009). The nuclear factor NF-kappaB pathway in inflammation. Cold Spring Harbor perspectives in biology, 1(6), a001651. https://doi.org/10.1101/cshperspect.a001651