Part:BBa_K203111
Constitutive promoter; 2 REU
A constitutive promoter from the Heidelberg 2009 library of promoters for use in mammalian cells.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
We generated a library of constitutive promoters by [http://2009.igem.org/Team:Heidelberg/Project_Synthetic_promoters RA-PCR]. We characterized those promoters by Flow cytometry.
This promoter corresponds to clone S10. We confirmed the initial measurement by an independet technique (image analysis). Its expression strength is approx. 2 [http://2009.igem.org/Team:Heidelberg/Project_Measurement REU].
MIT_MAHE 2020
Summary
NF-κB has been considered a prototypical proinflammatory signaling pathway. This is based on the activation of NF-κB by proinflammatory cytokines such as interleukin 1 (IL-1) and tumor necrosis factor α (TNFα). It also has a role in the expression of other proinflammatory genes including cytokines, chemokines, and adhesion molecules. This part contains a synthetic promoter upregulated by NF-κB activation. Manufactured by RA-PCR.
References
1. Lawrence T. (2009). The nuclear factor NF-kappaB pathway in inflammation. Cold Spring Harbor perspectives in biology, 1(6), a001651. https://doi.org/10.1101/cshperspect.a001651
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