Part:BBa_K4323000
Coding sequence for Aerolysin
Secreted, cytolytic toxin that forms pores in host membranes after proteolytic removal of a C-terminal propeptide, leading to destruction of the membrane permeability barrier and host cell death. The pores are formed by transmembrane beta-strands and are approximately 3 nm in diameter. This part has been codon optimized for expression in Escherichia coli. The composite part BBa_K4323001 includes the necessary components to express Aerolysin toxin. For more information, please visit our Wiki page.
Figure 1. The toxicity of Aerolysin to Dreissena bugensis.
Figure 2. His-Trap Ni-column purified Aerolysin. L: ladder, C5: 10 % B wash, D1: fraction of gradient at ~30 % B, D4: fraction of gradient at ~50 % B, D8: elution at 100 % B. A buffer: 20 mM Tris-HCl, 200 mM NaCl, pH 8.0; B buffer: 20 mM Tris-HCl, 200 mM NaCl, 300 mM imidazole, pH 8.0. Arrow: purified Aerolysin protein, MW: 55.2 kDa. Noted that 15 kDa and 10 kDa markers are not resolved.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 206
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 75
Illegal AgeI site found at 313
Illegal AgeI site found at 1270 - 1000COMPATIBLE WITH RFC[1000]
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