Composite

Part:BBa_K4323001

Designed by: Jessica Marinelli, Kevin Lim, Chun Hin Wong   Group: iGEM22_UManitoba   (2022-09-30)


Composite part to express Aerolysin toxin

This composite part includes the necessary components to express Aerolysin toxin (BBa_K4323000). It consists of a T7 promoter (BBa_I719005), a RBS (BBa_B0032), and terminator (BBa_B0015)

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 256
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 125
    Illegal AgeI site found at 363
    Illegal AgeI site found at 1320
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None