Regulatory

Part:BBa_K4192140

Designed by: Jiang Nanqi   Group: iGEM22_CAU_China   (2022-09-29)
Revision as of 15:17, 12 October 2022 by OwenDong (Talk | contribs)


PcysJH

A promoter consisting of PcysJ and PcysH in response to threonine concentration

Novel threonine sensing promoters including PcysJ and PcysH were discovered by proteomic analyses of E.coli in response to extracellular threonine challenges.

To enhance the strength of output signals, the promoters cysJp and cysHp were merged together to form a synthetic promoter cysJHp.

cysJp and cysHp could be affected by threonine independently.

Inducible activity of PcysJ and PcysH.png

Fig.1 Inducible activity of PcysJ, PcysH and PcysJH


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None