Regulatory

Part:BBa_K4192140

Designed by: Jiang Nanqi   Group: iGEM22_CAU_China   (2022-09-29)


PcysJH

A promoter consisting of PcysJ and PcysH in response to threonine concentration

Novel threonine sensing promoters including PcysJ and PcysH were discovered by proteomic analyses of E.coli in response to extracellular threonine challenges.

To enhance the strength of output signals, the promoters cysJp and cysHp were merged together to form a synthetic promoter cysJHp.

cysJp and cysHp could be affected by threonine independently.

Inducible activity of PcysJ and PcysH.png

Fig.1 Inducible activity of PcysJ, PcysH and PcysJH[1]


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[1]. Liu, Y.N., et al., Developing a high-throughput screening method for threonine overproduction based on an artificial promoter. Microbial Cell Factories, 2015. 14(1).


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