Part:BBa_K4156102
pLldR-Bxb1
pLldR-Bxb1 is constructed with L-lactate-sensitive promoter pLldR and serine integrase Bxb1, and is uesd for lactate-specific response for logic gate coupling.
Usage and Biology
pLldR-Bxb1 consists of a fusion of the lactate-sensitive promoter pLldR and the serine integrase Bxb1. It will act as a complex regulatory for controlling downstream logic gates and transcription of genes.
Characterization
In vitro characterization and data analysis of the reported strains
To improve signaling stability as well as accuracy, we added Amplifying genetic switches based on serine integrase (Bxb1,TP901) to the R reporter(BBa_ ) to construct the AR reporter. Fig 1 indicates lactate (plldR) induced AR reporter with homogenized fluorescence intensity (mRFP/Cell). Comparing Fig1, 2, it can be seen that the fluorescence intensity of the AR reporter decreased significantly at a lactate concentration of 0 mM, and its expression was more stable over time. The fluorescence intensity of the AR reporter was also greater at other concentrations of lactate induction, and the difference between the fluorescence intensity after lactate induction at each concentration was more pronounced. This result indicates that the addition of amplifying genetic switch enhances the reporter intensity and robustness of the lactate biosensor.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1479 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1753
Illegal XhoI site found at 1840 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 614
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2587
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