Regulatory

Part:BBa_K4156102

Designed by: Zheng Huang   Group: iGEM22_LZU-CHINA   (2022-10-07)


pLldR-Bxb1

pLldR-Bxb1 is constructed with L-lactate-sensitive promoter pLldR and serine integrase Bxb1, and is uesd for lactate-specific response for logic gate coupling.


Usage and Biology

pLldR-Bxb1 consists of a fusion of the lactate-sensitive promoter pLldR and the serine integrase Bxb1. It will act as a complex regulatory for controlling downstream logic gates and transcription of genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1479
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1753
    Illegal XhoI site found at 1840
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 614
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2587


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Categories
Parameters
None