Coding

Part:BBa_K4214000

Designed by: Jol Tekoniemi   Group: iGEM22_Stockholm   (2022-10-07)
Revision as of 07:35, 9 October 2022 by Zeniazen5 (Talk | contribs)

Human Gastric Intrinsic Factor (hGIF)


This part codes for human Gastric Intrinsic Factor. We used the human sequence of the gene. The gene is located in chromosome 11q12.1. This gene is a part of the cobalamin transporters group. It is essential for the absorption of vitamin B12. Mutations in this gene may lead to pernicious anemia.


Usage and Biology

  • NCBI page of the human gastric intrinsic factor [1]
  • We designed this biobrick in order to use this coding region for generating our mutants so that we ensure the evading of the autoantibodies of pernicious anemia
  • We used this part with some extra modifications to perform protein surface display in mammalian cells but also protein production in E.coli. These parts are BBa_K4214001 [2] , BBa_K4214002 [3], BBa_K4214003 [4], BBa_K4214004 [5].
  • This part of hGIF should be mentioned that does not contain the signal peptide


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 265
    Illegal BsaI.rc site found at 945
    Illegal SapI site found at 935
[edit]
Categories
//cds/membrane/transporter
Parameters
None