Composite

Part:BBa_K4115044

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-30)
Revision as of 06:57, 3 October 2022 by Yurj (Talk | contribs)


J23101-B0034-tagRFP-B0015

CMV:Ha-NLS-dCas9-Linker-VP16-NLS:BGH
Function gene reporter
Use in E.coli
RFC standard RFC 10, RFC 10 compatible
Backbone pBBR1
Submitted by (ShanghaiTech_China)

This composite part is used to express the tagRFP(BBa_K4115013) by a constitutive promoter J23101(J23101), thus we can use red fluorescence as a detection.

Proof of function

We transformed this plasmid into E. coli and encapsulated the E. coli into alginate gel beads. It was then induced to express tagRFP. We photograph the cross section of the gel beads under a confocal microscope and get Figure 1.

TagRFP in gel beads.jpg Figure 1: Confocal image of cross-section of gel beads with red fluorescent protein E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 695
    Illegal SapI.rc site found at 77


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Parameters
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