Composite
PcusC-GFP
Part:BBa_K4385012:Design
Designed by: Min An Zhao Group: iGEM22_Jilin_China (2022-10-01)
Copper sensor
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 826
Design Notes
This part is composed of ZraSR two-component signal transport system, PzraP and OmpC-ZBP. When the zinc ion concentration in the environment exceeds a certain concentration, the PzraP promoter promotes the transcription of downstream OmpC-ZBP, and realizes the adsorption of zinc ions in the environment. The 6*His Tag was added to this sequence to purify the recombinant protein.
Source
It comes from E. coli genomic DNA.