Composite
PcusC-GFP

Part:BBa_K4385012:Design

Designed by: Min An Zhao   Group: iGEM22_Jilin_China   (2022-10-01)


Copper sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 826


Design Notes

The copper sensor part is composed of PcusC and GFP. The initiation of the copper-inducible promoter PcusC requires the support of the CusSR two-component membrane associated sensor kinase system, which is present in the strain E. coli XL-1 Blue.

Source

It comes from E. coli genomic DNA.

References