Composite

Part:BBa_K4115040

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-30)
Revision as of 11:36, 3 October 2022 by Yurj (Talk | contribs)


J23101-B0034-LuxI-B0015

J23101-B0034-LuxI-B0015
Function gene regulator
Use in E.coli
RFC standard RFC 10, RFC 10 compatible
Backbone pBBR1
Submitted by (ShanghaiTech_China)

This composite part is used to produce 3OC6 HSL by LacI (BBa_C0161) and send it to the receiver protein LuxR (BBa_C0062)in the other E.coli.When the LuxR receives the signal, it will regulate and express the downstream report gene sfGFP, thus we can detect whether LuxI is functional by observing the fluorescence intensity under UV light.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None