Coding

Part:BBa_K4226002

Designed by: Siyu Han   Group: iGEM22_CAFA_China   (2022-09-19)
Revision as of 08:53, 26 September 2022 by Tigress K (Talk | contribs)


hrpS Gene

This part is an improvement for the part：HrpR Gene uploaded by iGEM13_HIT-Harbin, the hrpS Gene codes for hrpS protein. The hrpR (hrpR Gene) protein binds to hrpS (hrpS Gene) protein forming a complex, causing a transcriptionally upregulated of hrpL promoter(hrpL Promoter) .The Amp30E Amplification Device consists of hrpR, hrpS and hrpL promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 902
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 112
    Illegal SapI.rc site found at 745


[edit]
Categories
Parameters
None