Regulatory
pLacIQ1

Part:BBa_K091112:Design

Designed by: Pallavi Penumetcha   Group: iGEM08_Davidson-Missouri_Western   (2008-06-20)
Revision as of 21:28, 20 July 2009 by Alyndria.thompson (Talk | contribs) (→‎Design Notes)

pLacIQ1 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Although there is a 15 bp deletion in this promoter between the 27th and the 28th nucleotide versus the pLacIQ, there is also the addition of base pair at the 5' end of the sequence. This addition explains why both pLacIQ and pLacIQ1 have 56 bp total in their sequences, even with the 15 base pair deletion in pLacIQ1. [[Image:]]

Source

This sequence for the lacIQ1 promoter came from the paper Glascock and Weickert 1998.

References