Composite

Part:BBa_K4046620

Designed by: Ashley Jones   Group: iGEM21_Duke   (2021-10-21)
Revision as of 23:53, 21 October 2021 by Ayj9 (Talk | contribs)


hUBC-Kozak-cLuc

This is a constitutive reporter consisting of the hUBC promoter followed by the gene coding for Cypridina luciferase (cLuc), an enzyme that plays an important role in bioluminescence. cLuc that is expressed in cells can be secreted into the culturing media, which allows it to be used as a dynamic reporter for the concentration of the compound of interest. In between these two sequences is the Kozak sequence, which provides a start point for eukaryotic translation. This construct was added to a pcDNA5 plasmid and was created as one of our constitutive reporters; it will be used as a construct into which we insert our dhdO binding sequences in order to build a dynamic reporter for the compound D-2-HG.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1018
    Illegal BglII site found at 1077
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None