Part:BBa_K4046620
hUBC-Kozak-cLuc
Cypridina luciferase is an enzyme that plays an important role in bioluminescence. In order to do this, it cleaves its substrate, vargulin, which produces a luminescent molecule. cLuc that is expressed in cells can be secreted into the culturing media, which allows it to be used as a dynamic reporter for the concentration of the compound of interest. This reporter can be used as a luminescence-based constitutive reporter to quantitatively and non-invasively assess cell behavior.
To test the gene expression of hUBC-cLuc, we transfected a plasmid containing this construct into HEK293T cells, and cell media samples were tested for luciferase data after 2 days using vargulin. Overall, the results demonstrate that the cLuc gene behaves as expected, as it induces luminescence activity when acting on its vargulin substrate.
Figure 1: Luminescence assay results for cLuc gene with hUBC promoter
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1018
Illegal BglII site found at 1077 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |