DNA

Part:BBa_K3805209:Design

Designed by: Lijuan Wang   Group: iGEM21_BNU-China   (2021-09-30)
Revision as of 03:42, 22 October 2021 by YolandaYu (Talk | contribs)


Bi-stable Switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1287
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a toggle designed for the "guard" bacteria in our project. In the presence of AIP, the expression of mcherry-ompt will be activated. And it will be secreted outside the cell, stimulate the expression of ccdB and kill the "cheater" bacteria.In the absence of AIP, the toggle switch enables the "guard" bacteria to work like ordinary bacteria to produce the target protein.


Source

1.trc promoter：ptr99a plasmid 2.P2 promoter：https://parts.igem.org/Part:BBa_I746104 3.lacI- LVA：https://parts.igem.org/Part:BBa_C0012 4.mcherry-ompt:https://parts.igem.org/Part:BBa_K3805886

References