Primer
pT_tal-fwd

Part:BBa_K4047000:Design

Designed by: Hope Kirby, Avery Imes   Group: iGEM21_MiamiU_OH   (2021-09-01)
Revision as of 17:53, 4 October 2021 by Anniequyan (Talk | contribs) (→‎Design Notes)


primerT/TF_tal- forward


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 20
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 20
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 20
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 20
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 20
  • 1000
    COMPATIBLE WITH RFC[1000]


Generated from a combination of factors. Overlap sequence sourced from pAM2991. https://www.addgene.org/40248/ Annealing sequence sourced from Synechococcus elongatus PCC 7942 Genbank genome sequence for transaldolase, at the locus tag Synpcc7942_2297. https://www.ncbi.nlm.nih.gov/nuccore/CP000100.1?from=2367528&to=2368721&report=fasta&strand=2

References