Part:BBa_K3766022

T7 CGG promoter (reverse orientation)

This part is the T7 CGG promoter (reverse orientation). It is variant of the strong promoter from T7 bacteriophage (taatacgactcactata) with GG at 3' end.

Usage and Biology

The T7 CGG promoter is recognized specifically by the orthogonal T7 CGG-R12-KIRV RNA polymerase (BBa_K3766000) [1].

The T7 RNA polymerase initiates the transcription at the first guanidine of this stretch of three G and it was shown that +1 GG is one of the best +1, +2 base combinations at the transcription initiation for enhanced promoter strength [2].

T7 CGG promoter (reverse orientation)

References

[1] Meyer AJ, Ellefson JW, Ellington AD. Directed evolution of a panel of orthogonal T7 RNA Polymerase variants for in vivo or in vitro synthetic circuitry. ACS synthetic biology (2015) 4: 1070–1076.

[2] Imburgio D, Rong M, Ma K, McAllister WT. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry (2000) 39, 10419-10430.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 10
  
• 1000
  COMPATIBLE WITH RFC[1000]