Part:BBa_K3766022

T7 CGG promoter (reverse orientation)

This part is the T7_CGG promoter (TAATACCGGTCACTATA) in reverse orientation. It is a variant of the strong promoter from T7 bacteriophage (taatacgactcactata) with GG at 3' end.

Usage and Biology

The T7_CGG promoter is recognized specifically by the orthogonal T7 CGG-R12-KIRV RNA polymerase (BBa_K3766000) [1].

The T7 RNA polymerase initiates the transcription at the first guanidine of this stretch of three G and it was shown that +1 GG is one of the best +1, +2 base combinations at the transcription initiation for enhanced promoter strength [2].

References

[1] Meyer AJ, Ellefson JW, Ellington AD. Directed evolution of a panel of orthogonal T7 RNA Polymerase variants for in vivo or in vitro synthetic circuitry. ACS synthetic biology (2015) 4: 1070–1076.

[2] Imburgio D, Rong M, Ma K, McAllister WT. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry (2000) 39, 10419-10430.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 10
  
• 1000
  COMPATIBLE WITH RFC[1000]