Primer
pT_tal-fwd
Part:BBa_K4047000:Design
Designed by: Hope Kirby, Avery Imes Group: iGEM21_MiamiU_OH (2021-09-01)
primerT/TF_tal- forward
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 20
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 20
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 20
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 20
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 20
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
e
Generated from a combination of factors. Overlap sequence sourced from pAM2991. https://www.addgene.org/40248/ Annealing sequence sourced