Composite

Part:BBa_K3365054:Experience

Designed by: Zou Aixuan   Group: iGEM20_SJTU-BioX-Shanghai   (2020-10-22)
Revision as of 12:17, 26 October 2020 by Gyaxxxxxx (Talk | contribs) (→‎Applications of BBa_K3365054)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3365054

We add this part into off-target detection platform(part BBa_K3365062, BBa_K3365063, BBa_K3365064 and BBa_K3365065) as a reporter in the following three steps:

Firstly, we add target sequence to the head of spacer by conducting PCR-primer used in this procedure contains the target sequence. Secondly, we use Overlap PCR to connect these two fragments, for fear that target-blank fragment is too short to be attached to the eGFP sequence. Thirdly, we obtain terminator by performing PCR on pE1a-RFP and then link target+blank+eGFP and terminator through overlap PCR.

User Reviews

UNIQaa85b76a04f4a5d5-partinfo-00000000-QINU UNIQaa85b76a04f4a5d5-partinfo-00000001-QINU