Part:BBa_K3365054:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3365054

We add this part into the off-target detection platform(part BBa_K3365062, BBa_K3365063, BBa_K3365064 and BBa_K3365065) as a reporter by the following three steps:

Firstly, we add target sequence to the head of spacer by PCR: primers used in this procedure contains the target sequence. Secondly, we use Overlap PCR to connect target and eGFP fragment. The overlap PCR is used for fear that target-blank fragment is too short to be attached to the eGFP sequence. Thirdly, we obtain terminator by performing PCR on pE1a-RFP and then link target+blank+eGFP with terminator through overlap PCR.

User Reviews

UNIQ7d4d12db718f101e-partinfo-00000000-QINU UNIQ7d4d12db718f101e-partinfo-00000001-QINU