Part:BBa_K1933004

constitutive promoter and RBS

This part, consisting of BBa_J23105 (constitutive promoter) and BBa_B0034, was created only as a registry entry.

MIT_MAHE 2020

Summary

This is the single-chain variable fragments (scFv) of monoclonal antibodies Cetuximab. It is an epidermal growth factor receptor (EGFR) inhibitor, which specifically binds to the target antigen epidermal growth factor receptor (EGFR). With high affinity it can prevent ligand binding and activation of signal transduction.

References

Jiang, K., Li, J., Yin, J., Ma, Q., Yan, B., Zhang, X., Wang, L., Wang, L., Liu, T., Zhang, Y., Fan, Q., Yang, A., Qiu, X., & Ma, B. (2015). Targeted delivery of CXCR4-siRNA by scFv for HER2(+) breast cancer therapy. Biomaterials, 59, 77–87. https://doi.org/10.1016/j.biomaterials.2015.04.030

Kumar, M., Mani, P., Pratheesh, P., Chandra, S., Jeyakkodi, M., Chattopadhyay, P., Sarkar, D. P., & Sinha, S. (2015). Membrane Fusion Mediated Targeted Cytosolic Drug Delivery Through scFv Engineered Sendai Viral Envelopes. Current molecular medicine, 15(4), 386–400. https://doi.org/10.2174/1566524015666150505155949

De Jong, W. H., & Borm, P. J. (2008). Drug delivery and nanoparticles:applications and hazards. International journal of nanomedicine, 3(2), 133–149. https://doi.org/10.2147/ijn.s596

Deepagan, V. G., Sarmento, B., Menon, D., Nascimento, A., Jayasree, A., Sreeranganathan, M., Koyakutty, M., Nair, S. V., & Rangasamy, J. (2012). In vitro targeted imaging and delivery of camptothecin using cetuximab-conjugated multifunctional PLGA-ZnS nanoparticles. Nanomedicine (London, England), 7(4), 507–519. https://doi.org/10.2217/nnm.11.139

Fadaeian, G., Shojaosadati, S. A., Kouchakzadeh, H., Shokri, F., & Soleimani, M. (2015). Targeted Delivery of 5-fluorouracil with Monoclonal Antibody Modified Bovine Serum Albumin Nanoparticles. Iranian journal of pharmaceutical research : IJPR, 14(2), 395–405.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Usage

We used this part for construction of ☆BBa_K1933100, BBa_K1933101, BBa_K1933102, BBa_K1933103 ☆BBa_K1933200, and BBa_K1933201.