Coding

Part:BBa_K3629020

Designed by: Arshia Mostoufi   Group: iGEM20_Calgary   (2020-10-12)
Revision as of 07:37, 12 October 2020 by ArshiaMostoufi (Talk | contribs) (→‎Usage and Biology)


Yarrowia lipolytica TRP2 Gene

Arshia insert

Usage and Biology

2-isopropylmalate synthase is the first enzyme involved in the biosynthesis of leucine. This is a very important enzyme in the pathway as it serves as a regulatory point in the biosynthesis pathway. The protein has a leucine binding domain which allows for down regulation of the pathway through negative-feedback inhibition of the enzyme. Expression of this enzyme especially on a strong promoter could be used to upregulate leucine synthesis and produce a leucine overproducing strain.

Design

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 943
    Illegal NgoMIV site found at 1213
    Illegal NgoMIV site found at 1294
    Illegal NgoMIV site found at 1393
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 283
    Illegal BsaI.rc site found at 544
    Illegal BsaI.rc site found at 1441

References

[edit]
Categories
//awards/part_collection
//chassis/eukaryote/yeast
Parameters
None