Part:BBa_K3610001
BAK1 from Arabidopsis thaliana
This part contains the cDNA of the full-length plant cell surface receptor BAK1 from A. thaliana.
Usage and Biology
The BRI1-associated receptor kinase (BAK1) is a leucin-rich repeat receptor kinase (LRR-RK) which interacts with multiple other LRR-RKs with different functions in hormone signalling and defense response. BAK1 localizes at the plasma membrane and the endosome. The BAK1 protein forms a structure with an extracellular domain with leucin-rich repeats, a single pass transmembrane domain and an intracellular domain with a kinase function.
Among others, BAK1 interacts with the LRR-RKs EF-Tu receptor (EFR), Flagellin sensing 2 (FLS2) and cold-shock protein receptor (CORE), all of which are pathogen recognition receptors (PRR) in brassicaceae plants. Upon binding of a microbe-associated molecular pattern at the LRR domain of the PRR, BAK1 forms a heterodimer with the PRR which triggers a phosphorylation cascade, leading to upregulation of defense mechanisms.
In our project we used this receptor to demonstrate expression of plant PRRs in S. cerevisiae by expressing this part with a fluorescent marker fused to the N-terminal end of the sequence.
For characterization of the part see Part:BBa_K3610030
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1560
Illegal PstI site found at 650
Illegal PstI site found at 695
Illegal PstI site found at 985 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1560
Illegal PstI site found at 650
Illegal PstI site found at 695
Illegal PstI site found at 985 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1560
Illegal PstI site found at 650
Illegal PstI site found at 695
Illegal PstI site found at 985 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1560
Illegal PstI site found at 650
Illegal PstI site found at 695
Illegal PstI site found at 985 - 1000COMPATIBLE WITH RFC[1000]
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