Part:BBa_K523002

RBS + bglX (E. coli perisplasmic β-glucosidase)

This is the E. coli β-glucosidase gene bglX. The part contains the native Ribosome Binding Site.

The part was made using the strategy outlined in BBa_K523000, and therefore contains 4 extra bases at the 5' end which generate a BglII restriction site.

Usage and Biology

The product protein is believed to be periplasmic. β-glucosidase cleaves β(1→4) bonds, i.e. those found in the disaccharide cellobiose.

The SignalP program predicts that a 20 amino acid localisation signal (at the N terminal) is cleaved off before the protein reaches its mature form.

Edinburgh 2011 carried out some experiments on this part under the control of the lac promoter - see details at part BBa_K523014.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1658
  Illegal AgeI site found at 1880
  Illegal AgeI site found at 2069
  
• 1000
  COMPATIBLE WITH RFC[1000]

Functional Parameters: Austin_UTexas

BBa_K523002 parameters

Burden Imposed by this Part:

Burden Value: 2.0 ± 3.8%

Burden is the percent reduction in the growth rate of E. coli cells transformed with a plasmid containing this BioBrick (± values are 95% confidence limits). This BioBrick did not exhibit a burden that was significantly greater than zero (i.e., it appears to have little to no impact on growth). Therefore, users can depend on this part to remain stable for many bacterial cell divisions and in large culture volumes. Refer to any one of the BBa_K3174002 - BBa_K3174007 pages for more information on the methods, an explanation of the sources of burden, and other conclusions from a large-scale measurement project conducted by the 2019 Austin_UTexas team.

This functional parameter was added by the 2020 Austin_UTexas team.