Composite
Part:BBa_K3458003:Design
Designed by: Jiahua Zou Group: iGEM20_GDSYZX (2020-08-14)
35S Promoter + HQT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 191
Design Notes
- The goal of our team is to specifically express HQT, a key enzyme for chlorogenic acid synthesis in the endosperm of Oryza sativa L.. The promoter GluD-1 (BBa_K3458002) that we plan to use with specific expression characteristics in Oryza sativa L. endosperm cannot drive HQT express in rice protoplasts. In order to test our expression system in protoplasts, we designed a 35s promoter + HQT composite part for comparison with the GluD-1 promoter + HQT part (BBa_K3458004) .
Source
A