Part:BBa_K3458003:Design

35S Promoter + HQT

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 191
  

Design Notes

• The goal of our team is to specifically express HQT, a key enzyme for chlorogenic acid synthesis in the endosperm of Oryza sativa L.. The promoter GluD-1 (BBa_K3458002) that we plan to use with specific expression characteristics in Oryza sativa L. endosperm cannot drive HQT express in rice protoplasts. In order to test our expression system in protoplasts, we designed a 35s promoter + HQT composite part for comparison with the GluD-1 promoter + HQT part (BBa_K3458004) .

Source

• The HQT gene we used comes from the genome of Lonicera japonica (GeneBank: JF261014.1). We removed the restriction site incompatible with the biobrick assembly standard and optimized the codon according to the codon preference of Oryza sativa L..The HQT was synthesized in TIANYI HUIYUAN Company.

• The 35s promoter (BBa_K414002) has been registered by the iGEM10_Nevada team.

References

• Peng X, Li W, Wang W, et al. Cloning and characterization of a cDNA coding a hydroxycinnamoyl-CoA quinate hydroxycinnamoyl transferase involved in chlorogenic acid biosynthesis in Lonicera japonica[J]. Planta Med., 2010,76(16):1921-1926.