Plasmid_Backbone
Eukaryotic
Part:BBa_K157040
Designed by: iGEM Team Freiburg 2008 Group: iGEM08_Freiburg (2008-10-26)
Eukaryotic terminator vector (very similar to pSB1AK8)
Transfection vector with CMV Promotor for expression in human cells.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3405
Illegal XbaI site found at 3420
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3405
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 8
Illegal NotI site found at 3411 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3405
Illegal XhoI site found at 1272
Illegal XhoI site found at 2298 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a suffix.
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3405
Plasmid lacks a suffix.
Illegal XbaI site found at 3420
Illegal SpeI site found at 2
Illegal PstI site found at 16 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 2444
[edit]
Categories
Parameters
//chassis/eukaryote
//plasmidbackbone/operation
//plasmidbackbone/operation
chassis | |
copies | |
origin | pMB1 |
resistance | AK |