Composite

Part:BBa_K3269004

Designed by: Mark Liu   Group: iGEM19_Northwestern   (2019-10-12)
Revision as of 23:40, 21 October 2019 by Uroojamir (Talk | contribs)


LexA1 GFP composite part

This part includes having a LexA 1 operator upstream of GFP. This part was created with the intention of improving part BBa_K079050. For more information about our experience using this part, please view the experience page and the page for part BBa_K079050.

This part was meant to produce GFP proportionally to the amount of UV exposure. It was supposed to be a UV DNA damage sensor. However, it did not work as we anticipated. As our results in Figure 1 show, there was no significant difference in fluorescence level when the amount of UV changed.

T--Northwestern--lexA1data.png
Figure 1. Fluorescence/OD for different levels of UV for part BBa_K3269004 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 729


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