Part:BBa_K3269004

LexA1 GFP composite part

This part includes having a LexA 1 operator upstream of GFP. This part was created with the intention of improving part BBa_K079050. For more information about our experience using this part and how the LexA 1 operator compares to the LexA 2 operator, please view the experience page and the page for part BBa_K079050.

This part was meant to produce GFP proportionally to the amount of UV exposure. It was supposed to be a UV DNA damage sensor. However, it did not work as we anticipated. As our results in Figure 1 show, there was no significant difference in fluorescence level when the amount of UV changed. Figure 2 shows the difference in GFP fluorescence/OD for part BBa_K079050 and part BBa_K3269004.

Figure 1. Fluorescence/OD for different levels of UV for part BBa_K3269004

Figure 2. Fluorescence/OD600 for parts BBa_K3269004 (LexA1) and BBa_K079050 (LexA2). The time value next to each title is the exposure time for each sample

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 729